Rapid nanobody discovery and novel nanobody engineering s...
Nanobodies are recombinant single-domain antibodies of camelid origin. We set out to generate nanobodies as tools to study nuclear pore complexes (NPCs) that constitute the major passageways for nucleocytoplasmic exchange. Therefore, we developed an optimized phage display-based workflow for the generation of nanobodies from an immunized animal. The identified nanobodies were functionally expressed in the E. coli cytoplasm in a ...